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KMID : 1094720220270060961
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Biotechnology and Bioprocess Engineering
2022 Volume.27 No. 6 p.961 ~ p.974
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Curcumin Stimulates UCP1-independent Thermogenesis in 3T3-L1 White Adipocytes but Suppresses in C2C12 Muscle Cells
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Choi Min-Ji
Sulagna Mukherjee
Yun Jong-Won
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Abstract
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Non-shivering thermogenesis may be an effective way to alter the energy balance in adipocytes and skeletal muscle. Curcumin stimulates adipocyte browning and improves the mitochondrial function in adipocytes via uncoupling protein 1 (UCP1)-dependent thermogenic activity. On the other hand, the UCP1-independent thermogenic effect of curcumin on adipose tissues and muscle remains unexplored. This study examined whether curcumin can also induce UCP1-independent thermogenesis in 3T3-L1 white adipocytes and C2C12 muscle cells. Curcumin stimulated the expression of α1-adrenergic receptor (α1-AR), UCP1-independent thermogenic markers, sarcoendoplasmic reticulum Ca2+-ATPase (SERCA) 2b, and ryanodine receptor 2 in adipocytes, whereas it suppressed SERCA/sarcolipin-based thermogenesis in muscle cells. Curcumin stimulated non-shivering thermogenesis by activating mitochondrial uncoupling and the SERCA/SLN axis in white adipocytes but not muscle cells. In addition, curcumin stimulated thermogenesis by activating the creatine metabolism-related thermogenic pathway in white adipocytes. Taken together, curcumin induces UCP1-independent creatine-mediated and α1-AR/SERCA-based thermogenesis through ATP-consuming futile processes. Together with previous results, the anti-obesity effect of curcumin involves mainly two interacting parts, one mediated via β3-AR and cAMP (UCP1-dependent) and the other via α1-AR and increase in cytosolic Ca2+ levels (UCP1-independent) in beige fat but not in muscle cells.
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KEYWORD
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curcumin, C2C12 muscle cells, obesity, 3T3-L1 adipocytes, UCP1-independent thermogenesis
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